Product Name: Human Protein Kinase B ,PKB ELISA Kit

• Wavelength: 450nm
• Sample Volume: 10ul

Purpose

Our Human protein kinase B,PKB ELISA Kit is to assay PKB levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to PKB. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PKB is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PKB and HRP conjugated PKB antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wave length of 450 nm. The OD value is proportional to the concentration of PKB. You can calculate the concentration of PKB in the samples by comparing the OD of the samples to the standard curve.