With L-Glutamine Without Tryptose Phosphate broth and Sodium bicarbonate
Glasgow’s Minimum Essential Medium (GMEM) is a modified version of Basal Medium Eagle (BME), developed by Ian Macpherson and Michael Stoker. They enhanced the original formulation by adding tryptose phosphate broth and doubling the concentrations of amino acids and vitamins.
| Ingredients - INORGANIC SALTS | |
| Calcium chloride dihydrate | 265.00 |
| Ferric nitrate nonahydrate | 0.100 |
| Magnesium sulphate anhydrous | 97.72 |
| Potassium chloride | 400.00 |
| Sodium chloride | 6400.00 |
| Sodium dihydrogen phosphate monohydrate | 109.00 |
| AMINO ACIDS | |
| L-Cystine | 24.00 |
| L-Glutamine | 292.0 |
| L-Arginine hydrochloride | 42.00 |
| L-Histidine hydrochloride-H2O | 21.00 |
| L-Isoleucine | 52.40 |
| L-Leucine | 52.40 |
| L-Lysine hydrochloride | 73.10 |
| L-Methionine | 15.00 |
| L-Phenylalanine | 33.00 |
| L-Threonine | 47.60 |
| L-Tryptophan | 8.00 |
| L-Tyrosine disodium salt | 52.00 |
| L-Valine | 46.80 |
| VITAMINS | |
| Choline chloride | 2.00 |
| D-Calcium pantothenate | 2.00 |
| Folic Acid | 2.00 |
| Niacinamide | 2.00 |
| Pyridoxal hydrochloride | 2.00 |
| Thiamine hydrochloride | 2.00 |
| Riboflavin | 0.200 |
| i-Inositol | 3.60 |
| OTHERS | |
| D-Glucose | 4500.00 |
| Phenol red sodium salt | 15.00 |
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