Lipid Peroxidation MDA Assay Kit uses a color reaction based on the reaction of MDA and thiobarbituric acid (TBA) to produce red product. It is used to determine MDA in plasma, serum, urine, animal and plant tissue or cell lysate by colorimetry. It is widely used as a test kit to detect lipid peroxidation level.

Malondialdehyde (MDA) is a natural product of lipid oxidation. Lipid oxidation occurs when oxidative stress occurs in animal or plant cells. Some fatty acids are oxidized and gradually broken down into a range of complex compounds, including MDA. At this time, the level of lipid oxidation can be detected by detecting the level of MDA, so the determination of MDA is widely used as an indicator of lipid oxidation. MDA is also produced by some other biochemical reactions in vivo, such as thromboxane synthase, but changes in lipid oxidation levels can be observed as long as appropriate controls are set during measurement.

Malondialdehyde can react with TBA in high temperature and acidic environment to form the red MDA-TBA adduct. The corresponding reaction principle diagram is as follows:

The MDA-TBA adduct has a maximum absorption at 535nm, which can be detected by colorimetry. In addition, the MDA-TBA adduct can also be excited at 535nm to produce a maximum emission wavelength of 553nm, according to which fluorescence detection can also be performed.

Note: Aldehydes and relatively high concentrations of soluble sugars (such as 250mM sucrose) interfere with the reaction, and the products of soluble sugar and TBA color reaction are also absorbed at 532nm (the maximum absorption is 450nm).If soluble sugar interferes with the determination, dual-wavelength determination can be performed by measuring 450nm as a reference wavelength to eliminate the interference.