BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium) Substrate Solution is a high-quality chromogenic substrate for alkaline phosphatase (AP) enzyme detection, widely used in molecular biology, immunology, and biochemical assays. When reacted with alkaline phosphatase, this substrate produces an intense purple precipitate, ensuring clear and reliable visual detection of target proteins and nucleic acids.

Ideal for Western blotting, ELISA, and in situ hybridization, the BCIP/NBT Substrate Solution is a ready-to-use, stable formulation designed for high sensitivity and consistent performance in AP-based detection systems. In this reaction, BCIP serves as the substrate while NBT acts as the color development reagent, resulting in a durable purple color that provides excellent contrast and precise visualization for accurate analytical results.