This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with T. T in the sample or standard competes with a fixed amount of biotin-labeled T for sites on a pre-coated antibody specific to T. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of T in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
Specification
| Product Name | Human T (Testosterone) ELISA Kit |
| Alias | Testosterone ELISA Kit, T ELISA Kit |
| UniProt ID | CAS:58-22-0 |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with T , no obvious cross reaction with other analogues. |
Standard Curve
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
| STD.(pg/ml) | OD-1 | OD-2 | Average |
| 0 | 2.133 | 2.242 | 2.177 |
| 31.25 | 1.843 | 1.937 | 1.881 |
| 62.5 | 1.537 | 1.615 | 1.568 |
| 125 | 1.327 | 1.395 | 1.354 |
| 250 | 0.96 | 1.009 | 0.98 |
| 500 | 0.645 | 0.678 | 0.658 |
| 1000 | 0.453 | 0.476 | 0.462 |
| 2000 | 0.265 | 0.278 | 0.27 |
Recovery
Add a certain amount of T into the sample. Calculate the recovery by comparing the measured value with the expected amount of T in the sample.
| Sample Type | Recovery Range(%) | Average Recovery(%) |
| serum(n=10) | 89-101 | 94 |
| EDTA plasma(n=10) | 85-104 | 98 |
| Heparin plasma(n=10) | 86-102 | 96 |
Linearity
Dilute the sample with a certain amount of T at 1:2, 1:4 and 1:8 to get the recovery range.
| Sample Type | 1:2 | 1:4 | 1:8 |
| serum(n=10) | 98-103% | 98-104% | 91-105% |
| EDTA plasma(n=10) | 84-101% | 89-100% | 82-99% |
| Heparin plasma(n=10) | 83-98% | 85-100% | 84-95% |
Precision(%)
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item | Intra-assay Precision | Inter-assay Precision | ||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/ml) | 58.4 | 233.7 | 975.1 | 60.28 | 242.4 | 963 |
| Standard deviation | 2.85 | 11.47 | 50.02 | 2.91 | 12.22 | 49.21 |
| CV(%) | 4.88 | 4.91 | 5.13 | 4.82 | 5.04 | 5.11 |
Stability
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
| ELISA kit(n=5) | 37°C for 1 month | 2-8°C for 6 months |
| Average(%) | 80 | 95-100 |
Reviews
There are no reviews yet.