The HyPure Universal DNA Kit is designed for purification of genomic DNA from a wide range of biological samples using silica membrane spin column technology. The optimized extraction chemistry supports efficient DNA recovery from complex sample matrices including blood, tissue, buffy coat, body fluids, swabs, dried blood spots and sperm samples.
The kit combines Proteinase K digestion with silica membrane adsorption to efficiently release genomic DNA from biological samples while removing proteins, lipids and other contaminants. The resulting DNA is suitable for routine molecular biology workflows such as PCR amplification, genotyping analysis and HLA typing studies.
DNA extraction portfolio, the HyPure Universal DNA Kit is positioned as a practical column-based solution for laboratories handling mixed sample types in routine workflows. Rather than being optimized for a single matrix, it provides a unified purification route across multiple common sample types, which is useful when workflow simplicity and broad sample compatibility are both required.
Workflow Overview
The HyPure Universal DNA Kit uses a silica column-based workflow for purification of total DNA from routine sample types including tissue, blood, body fluids, cultured cells and swab-based materials. After sample-specific pretreatment, DNA is released by protease-assisted lysis, captured on the silica membrane under ethanol-containing binding conditions, and recovered through washing, drying and elution.
Sample Handling Logic
The main variation between sample types lies in the pretreatment and digestion stage. Tissue samples require cutting and complete digestion, cultured cells require pellet resuspension before lysis, while blood and other liquid samples can usually enter lysis more directly. Once the binding conditions are established, the downstream purification steps remain largely consistent.
Time and Workflow Characteristics
Under typical manual operation, the overall workflow for routine samples is usually completed within about 30–90 minutes, depending mainly on sample pretreatment and digestion requirements.
| Specifications | |
| Features | Specifications |
| Main Functions | Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots |
| Applications | PCR, qPCR, southern bolt and virus detection, etc |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples |
| Sample amount | Solid tissue: 1-10mg; Anticoagulant blood: 200μl |
| Elution volume | ≥20μl |
| Time per run | 30 - 90 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Typical Applications
Genomic DNA purified using the HyPure Universal DNA Kit is suitable for a wide range of molecular biology and molecular diagnostics research workflows, including:
- PCR amplification
- Genotyping analysis
- HLA typing studies
- Molecular diagnostics research
- Population genetics studies
- Sample preparation for sequencing workflows
- Biobanking sample processing
| Kit Contents | ||
| Contents | D318102 | D318103 |
| Purification Times | 50 | 250 |
| HyPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 100 | 500 |
| Buffer ATL | 30 ml | 150 ml |
| Buffer AL | 30 ml | 150 ml |
| Buffer GW1 | 22 ml | 88 ml |
| Buffer GW2 | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer AE | 15 ml | 60 ml |
