Dulbecco’s Modified Eagle-High Glucose (DMEM) Powder

Dulbecco's Modified Eagle-High Glucose (DMEM) Powder- With 4 mM L-alanyl-L-glutamine, 1 mM sodium pyruvate, phenol red indicator, without HEPES
Product Name Dulbecco's Modified Eagle-High Glucose (DMEM) Powder
Catalogue No CMP8511-1L
Quantity 13.9g of powder per litre
Storage 2-8° C
Shipping condition Ambient
Self-life 36 months from the date of manufacturing
Application DMEM/High Glucose is widely used in cells with fast growth and low adhesion.
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DMEM/High Glucose, i.e., DMEM (Dulbecco’s Modified Eagle Medium), is improved based on MEM Medium, and has been widely used in the culture of various cells. This product contains amino acids, vitamins, inorganic salts, and other components necessary for cell culture, without protein or growth factor.

Ingredients - INORGANIC SALTS
Calcium chloride dihydrate 200.00
Ferric nitrate nonahydrate 0.100
Magnesium sulphate anhydrous 97.67
Potassium chloride 400.00
Sodium chloride 6400.00
Sodium dihydrogen phosphate monohydrate 109.00
AMINO ACIDS
Glycine 30.00
L-Alanine 84.00
L-Arginine hydrochloride 147.50
L-Cystine dihydrochloride 62.60
L-Histidine hydrochloride-H2O 42.00
L-Isoleucine 105.00
L-Leucine 105.00
L-Lysine hydrochloride 146.00
L-Methionine 30.00
L-Phenylalanine 66.00
L-Serine 40.00
L-Threonine 95.00
L-Tryptophan 16.00
L-Tyrosine disodium salt 103.79
L-Valine 94.00
L-Alanyl-L-Glutamine 868.90
VITAMINS
Choline chloride 4.00
D-Calcium pantothenate 4.00
Folic Acid 4.00
Niacinamide 4.00
Pyridoxal hydrochloride 4.00
Thiamine hydrochloride 4.00
Riboflavin 0.400
i-Inositol 7.20
OTHERS
D-Glucose 4500.00
Phenol red sodium salt 14.95
Sodium pyruvate 110.00

 

  • Suspend the pouch content in 900 ml of ultra-pure water or water for injection grade suitable for tissue culture with constant stirring until the powder is completely dissolved
  • Weigh 3.7 g of sodium bicarbonate powder and add to 1 litre of medium and stir until dissolved
  • Adjust the pH to ~0.25 (pH 7.0 to 7.4) below the desired pH using 1N HCl or 1N NaOH, since the pH tends to rise during filtration process.
  • Make up the volume to 1000ml with water
  • Sterilize filter through a sterile membrane filter with a porosity of 0.22μm, using positive pressure to avoid the loss of carbon dioxide
  • Aseptically add sterile supplements as required and aliquot sterile medium into sterile cell culture grade bottles
  • Store liquid medium at 2-8°C till further use

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