| Biological Source | Goat |
| Conjugate | Peroxidase Conjugate |
| Antibody Form | Affinity Isolated Antibody |
| Antibody Product Type | Secondary Antibodies |
| Clone | Polyclonal |
| Species Reactivity | Rabbit |
| Should not react with | Human |
| Technique(s) | direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL) |
| Shipped in | Dry Ice |
| Storage Temp. | −20°C |
| Immunogen | Purified mouse IgG |
| Target Post-Translational Modification | Unmodified |
| Immunogen | Purified Rabbit IgG |
| Manufacture By | Sigma-Aldrich Solutions |
General Description
>Goat anti-rabbit IgG-peroxidase conjugate associates with all rabbit Igs. The conjugate shows no reaction with human IgG by immunoelectrophoretic (IEP). Furthermore, the antibody is adsorbed with human IgG to reduce background staining with human samples.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one inter heavy disulfide bridge. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Specificity
Specificity of the Anti-Rabbit IgG-Peroxidase is determined by immunoelectrophoretic (IEP) using normal rabbit serum and rabbit IgG. The conjugate shows no reaction with human IgG by IEP.
Application
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunofluorescence staining , immunochemistry and immunoprecipitations. Co-immunoprecipitation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody. Goat anti-rabbit IgG conjugated to peroxidase can be used for direct ELISA and immunohistochemistry.
Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution and yeast lysates following protein carbonylation assays at a dilution of 1:5000
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat has been used as a secondary antibody during immunofluorescence staining.
Biochem/Physiol Actions
IgGs (immunoglobulins) modulate several immune responses. It mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by two-step glutaraldehyde method.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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