| Appearance (Form) | Liquid |
| Appearance (Colour) | Dark Blue |
| Appearance (Clarity) | Clear |
| Concentration | 2X |
| pH of solution | 6.8 |
| SDS-PAGE Analysis | Good resoulution of individual proteins and optimum visibility of tracking dye during electrophoresis. |
| Manufacturer | Sisco Research Laboratories |
Description
A ready-to-use 2X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer.
Role of 2X SDS PAGE Sample buffer :
- Tris-HCl is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart.
- Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge.
- The high density of glycerol ensures the sample moves down into the well.
- Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes.
- Â The dye visually indicates the location (tracking) of the sample in the gel.