Anti-Mouse IgG (whole molecule)–Peroxidase Antibody

Synonym(s) Anti Mouse Hrp Sigma
Catalogue No A4416
Reactivity Mouse
Biological Source Goat
Applications Research Pathology
Clonality Polyclonal
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SKU: A4416 Categories: ,
Form Buffered Aqueous Solution
Conjugate Peroxidase Conjugate
Antibody Form Affinity Isolated Antibody
Antibody Product Type Secondary Antibodies
Technique(s) Direct ELISA: 1:10,000
Shipped in Dry Ice
Storage Temp. −20°C
Immunogen Purified mouse IgG
Target Post-Translational Modification Unmodified
Manufacture By Sigma-Aldrich Solutions

General Description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for mouse IgGs.

Application

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot (1:2000). The product can also be used for direct ELISA: 1:10,000.
Western blot analysis of transfected Hela cell lysates was performed using HRP conjugated goat anti-mouse IgG as the secondary at a dilution of 1:4000.[1]

Physical Form: Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms GHS07
Signal Word Warning
Hazard Statements H317,H412
Precautionary Statements P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
Hazard Classifications Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code 12 - Non Combustible Liquids
WGK Mouse
Flash Point(F) Not applicable
Flash Point(C) Not applicable

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