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Rat REN(Renin) ELISA Kit

Biostring > Products > Rat REN(Renin) ELISA Kit
Rat REN(Renin) ELISA Kit

Rat REN(Renin) ELISA Kit

Catalogue No. RE1064R
Size 48T/96T
Range 15.63-1000pg/mL
Sensitivity 9.38 pg/mL
Reactivity Rat
Sample Serum, Plasma, Tissue homogenate and Other biological samples；

Sample Volume=100μL
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Description

Alternative Names REN1, HNFJ2, renin-1
Detection Method Sandwich
Storage 2-8°C(Sealed), Don’t cryopreserve.
Research Area Metabolic pathway; Endocrinology; Hormone metabolism;
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat REN. You can calculate the concentration of Rat REN in the samples by comparing the OD of the samples to the standard curve.
Precautions The product listed herein is for research use only and not intended for diagnosis field. The international literature is also constantly updated, it is best to confirm with the salesman or email us before ordering. The product listed herein all was produced by our laboratory. If you have any other requirements, please email us, to get you wanted product information.
Documents Product manual with the material

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD Corrected
1000.00 2.457 2.430
500.00 1.577 1.550
250.00 0.884 0.857
125.00 0.449 0.422
62.50 0.240 0.213
31.25 0.134 0.107
15.63 0.083 0.056
0.00 0.027 0.000

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 21.36 212.71 302.00 30.44 154.14 342.42
Standard deviation 1.53 7.04 18.06 0.91 9.06 15.74
C V (%) 4.34 3.87 7.47 7.63 3.59 7.41

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 80-94 87
EDTA plasma (n=8) 95-107 101
Cell culture media (n=8) 82-95 88

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-102 90-99 82-97
Average (%) 93 97 86
1:4 Range (%) 93-105 90-99 88-103
Average (%) 101 91 103
1:8 Range (%) 91-99 95-106 83-94
Average (%) 98 106 84
1:16 Range (%) 83-96 87-101 92-105
Average (%) 91 100 100

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