DMEM/High Glucose, i.e., DMEM (Dulbecco’s Modified Eagle Medium), is improved based on MEM Medium, and has been widely used in the culture of various cells. This product contains amino acids, vitamins, inorganic salts, and other components necessary for cell culture, without protein or growth factor. With 4 mM L-alanyl-L-glutamine, 1 mM sodium pyruvate, phenol red indicator, without HEPES.
Specification
| Ingredients | MG/ L |
| INORGANIC SALTS | |
| Calcium chloride dihydrate | 200.00 |
| Ferric nitrate nonahydrate | 0.100 |
| Magnesium sulphate anhydrous | 97.67 |
| Potassium chloride | 400.00 |
| Sodium chloride | 6400.00 |
| Sodium dihydrogen phosphate monohydrate | 109.00 |
| AMINO ACIDS | |
| Glycine | 30.00 |
| L-Alanine | 84.00 |
| L-Arginine hydrochloride | 147.50 |
| L-Cystine dihydrochloride | 62.60 |
| L-Histidine hydrochloride-H2O | 42.00 |
| L-Isoleucine | 105.00 |
| L-Leucine | 105.00 |
| L-Lysine hydrochloride | 146.00 |
| L-Methionine | 30.00 |
| L-Phenylalanine | 66.00 |
| L-Serine | 40.00 |
| L-Threonine | 95.00 |
| L-Tryptophan | 16.00 |
| L-Tyrosine disodium salt | 103.79 |
| L-Valine | 94.00 |
| L-Alanyl-L-Glutamine | 868.90 |
| VITAMINS | |
| Choline chloride | 4.00 |
| D-Calcium pantothenate | 4.00 |
| Folic Acid | 4.00 |
| Niacinamide | 4.00 |
| Pyridoxal hydrochloride | 4.00 |
| Thiamine hydrochloride | 4.00 |
| Riboflavin | 0.400 |
Note: It is recommended to monitoring the pH of the medium. If needed, pH can be adjusted by using sterile 1N HCl or 1N NaOH.
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