|
E2 (Estradiol) ELISA Kit |
|
Sensitivity |
7.5 pg/mL |
|
Range |
12.5-800 pg/mL |
|
Detection method |
Competitive |
|
Standard |
800pg/mL |
|
Sample volume |
50μL |
|
Sample type |
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids |
|
Reaction time |
1.5H |
|
Research Area |
Signaling transduction；Cancer；Metabolism |
|
Alternative Names |
Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol |
|
Principle |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to E2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve |
|
Validated Image |
|
