The 5X RNA Loading Buffer is formulated for loading RNA samples on agarose gels. This buffer contains two tracking dyes: bromophenol blue and xylene cyanol. Bromophenol blue migrates faster than 5S rRNA and xylene cyanol migrates slower than 18S rRNA. This solution does not mask RNA in the gel during exposure to UV light. This dye solution contains the denaturing agent formamide and as a result all the RNA samples are separated according to their size. Moreover, formamide stabilizes the RNA .

Electrophoresis Test

Dye in 1X concentration is mixed with RNA sample and the mobility is tested by Agarose gel electrophoresis.

Stability