| Feature | Specifications |
| Recommended application | Plasmid Maxi preparation |
| Preservation conditions | Room temperature |
| Stability | Up to 4 years |
| Filter membrane | High quality glass fiber filter GF/B, 8 layers |
| Membrane aperture | 1.0 μm |
| Maximum binding yield of plasmid | 1 MG |
| Maximum yield of alcohol mediated Binding | 5 MG |
| Plasmid yield | Up to 1MG |
| Single liquid carrying capacity of column | 20 ML |
| Minimum elution volume | 800 μl |
| Withstand centrifugal force | 3,000-5,000 x g |
| Centrifuge | Low speed centrifuge for 50ml centrifuge tubes, >3000 x g, swing-out Rotor |
BioString™ DNA Maxi Column III
| Catalogue No | BS-DNA-059 |
| Product Name | BioString™ DNA Maxi Column III |
| Size | 100/Bag |
| Price | POR |
This column is used for plasmid preparation at the largest scale within the column system. It provides sufficient binding capacity to support high-yield plasmid workflows from bulk cultures.
Compared with midi formats, it is better suited for applications where DNA yield is prioritized over processing speed.
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.