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BioString™ gDNA Maxi Column

BioString™ gDNA Maxi Column

Catalogue No BS-DNA-058
Product Name BioString™ gDNA Maxi Column
Size 100/Bag
Price POR
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Feature Specifications
Recommended application gDNA maxi yield preparation, total RNA maxi preparation
Preservation conditions Room temperature
Stability Up to 4 years
Filter membrane High quality glass fiber filter GF/B, 4 layers
Membrane aperture 1.0μm
Maximum binding yield of plasmid 500 μg
Maximum yield of alcohol mediated Binding 5 mg
gDNA or RNA yield (>30%ethanol) Up to 5mg
Single liquid carrying capacity of column 20 ml
Minimum elution volume 800 μl
Withstand centrifugal force 3,000~5,000 x g
Centrifuge Low speed centrifuge for 50mlcentrifuge tubes, >3000 x g, swing-out Rotor

The Maxi Column is used for high-input workflows where sample volume and expected yield exceed the capacity of midi systems. It supports large-volume loading while maintaining stable adsorption performance.

This format is commonly used in workflows requiring milligram-scale DNA recovery from bulk biological material.

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.