| Trypsin Sequencing Grade, Modified from bovine pancreas | |
| Synonym Name | Trypsin |
| Biological source | Bovine Pancreas |
| Concentration | 0.01-0.2 % (w/w) |
| form | lyophilized (salt-free) |
| technique(s) | protein sequencing: suitable |
| impurities | Chymotrypsin |
| color | white |
| optimum pH | 8.0 |
| solubility | 10 g/L |
| suitability | suitable for protein modification |
| UniProt accession no. | P00760 |
| application(s) | life science and biopharma |
| foreign activity | Contaminating activities corresponds, Chymotrypsin , contains |
| storage temp. | 2-8°C |
| Gene Information | cow PRSS1(780933) |
| Manufacturer | Sigma-Aldrich® Solutions |
Trypsin Sequencing Grade, Modified from Bovine Pancreas
| Catalogue No | 11418025001 |
| Mol. Formula | - |
| Mol. Weight | 24.000 g/mol |
| Price | POR |
Trypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified. Trypsin is a highly efficient and specific protease widely used in proteomics for protein digestion. It produces short peptides with specific characteristics that are compatible with current separation and identification methods such as liquid chromatography, mass spectrometry (MS)
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.
Use Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins.
It is used for:
- Protein-structure elucidation
- Tryptic mapping
- Fingerprinting analysis
- Sequence analysis
- Translocation studies
- Protein identification
- Protein digestion during lipoprotein preparation for liquid chromatography-tandem mass spectrometry (LC-MS/MS)