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N-Myc/MYCN Rabbit mAb

N-Myc/MYCN Rabbit mAb

Catalogue No BSAB-355P
Host Rabbit
Reactivity Human, Mouse, Rat
Applications WB, IP, IF/ICC, IHC-P, ChIP, ELISA
Size 100 µL
Price POR
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SKU: BSAB-355P Categories: ,
N-Myc/MYCN Rabbit mAb
Observed MW 57-65 kDa
Calculated MW 50 kDa
Category Monoclonal Antibodies
Conjugation Unconjugated
Clone number ARC3826
Swiss Prot P01498/P01106
Gene ID 4613/4609
Synonyms NMYC; ODED; MODED; MPAPA; N-myc; FGLDS1; bHLHe37; MYCNsORF; MYCNsPEP
Purification Affinity purification
Isotype IgG
Form Liquid
Cellular Localization Nucleus
Positive Sample IMR-32,Baf3
Recommended Dilution WB 1:10000 - 1:50000(Human);1:3000 - 1:7000(Mouse), IF/ICC 1:200 - 1:1400, ChIP 3 μg antibody for 15 μg - 20 μg of Chromatin, ELISA: Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (≥1:10000),a sequential dilution method is strongly recommended to ensure measurement accuracy.
Background This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene
Validated Image

Immunohistochemistry analysis of paraffin-embedded Mouse brain using N-Myc/MYCN Rabbit pAb (A0499) (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunofluorescence analysis of paraffin-embedded rat brain using N-Myc/MYCN Rabbit pAb (A0499) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.