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Human TLR4(Toll-like receptor 4) ELISA Kit |
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Sensitivity |
0.188ng/ml |
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Range |
0.313-20ng/ml |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Alternative Names |
Toll-like receptor 4 ELISA Kit, hToll ELISA Kit, TLR4 ELISA Kit |
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Assay length |
4 hours |
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Detection Wavelength |
OD450 |
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Uniprot ID |
O00206 |
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Specificity |
Specifically binds with TLR4 , no obvious cross reaction with other analogues. |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TLR4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TLR4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TLR4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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