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Human Hepc (Hepcidin) ELISA Kit |
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Sensitivity |
18.75pg/ml |
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Range |
31.25-2000pg/ml |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Alternative Names |
Hepcidin ELISA Kit, Liver-expressed antimicrobial peptide 1 ELISA Kit, LEAP-1 ELISA Kit, Putative liver tumor regressor ELISA Kit, PLTR ELISA Kit, Hepcidin-25 ELISA Kit, Hepc25 ELISA Kit, Hepcidin-20 ELISA Kit, Hepc20 ELISA Kit, HAMP ELISA Kit, HEPC ELISA Kit, LEAP1 ELISA Kit, UNQ487 ELISA Kit, PRO1003 ELISA Kit |
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Assay length |
4 hours |
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Detection Wavelength |
OD450 |
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Uniprot ID |
P81172 |
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Specificity |
Specifically binds with Hepc , no obvious cross reaction with other analogues. |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Hepc antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Hepc bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Hepc in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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