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Human HMGB-1(High Mobility Group Protein B1) ELisa Kit |
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Sensitivity |
26.9 pg/mL |
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Range |
62.5-4000 pg/mL |
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Alternative Name |
HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1 |
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UniProt ID |
P09429 |
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Sample Type |
serum, plasma and other biological fluids |
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Detection Method |
Sandwich |
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Standard |
4000 pg/mL |
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Reaction Duration |
3.5 hours |
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Research Area |
Infection immunity |
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Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Validated Image |
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