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Human Cholecystokinin-8 (CCK) Elisa Kit |
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Sensitivity |
< 4.22pg/ml |
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Range |
12.35-1,000pg/ml |
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Accuracy & Precision |
Intra-Assay: CV<10% Inter-Assay: CV<15% |
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Sample Type |
Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
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Detection Method |
Sandwich |
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Storage |
Shipped at 2-8°C. Longterm Storage at -20° C |
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Description |
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CCK8 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
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Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to CCK8 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CCK8 and unlabeled CCK8 (Standards or samples) with the pre-coated antibody specific to CCK8. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CCK8 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CCK8 in the sample. |
