| Rapid cDNA Synthesis Kit | |
| Function | First-strand cDNA synthesis from RNA for RT-PCR and real-time RT-PCR |
| Enzyme system | Uses M-MuLV Reverse Transcriptase with RNase inhibitor in an optimized master mix |
| Primers | Includes oligo(dT) and random hexamer primers |
| Target size | Optimized for synthesis of cDNA targets <1 kb |
| Performance | Produces high-quality cDNA suitable for both conventional and real-time PCR |
| Purity | ≥95% purity of M-MuLV Reverse Transcriptase confirmed by 10% reducing SDS-PAGE Unit definition. One unit incorporates 1 nmol of dTTP into acid-insoluble form in 10 minutes at 37 °C using poly(rA):oligo(dT) as template–primer |
| Storage | Store at –20 °C |
| Handling note | Spin tubes briefly before use |
Rapid cDNA Synthesis Kit
| Catalogue No | BS-RTPCR-001 |
| Product | Rapid cDNA Synthesis Kit (Master mix based) |
| Type | Recombinant (rDNA product) |
| Size | 25 Reactions | 50 Reactions | 100 Reactions |
| Price | POR |
cDNA synthesis kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit consists of two optimized mixes, M-MuLV Enzyme Mix and M-MuLV Reaction Mix. M-MuLV Enzyme Mix combines M-MuLV Reverse Transcriptase and Murine RNase Inhibitor, while M-MuLV Reaction Mix contains dNTPs, buffer and an optimized blend of oligo(dT) and random hexamer primers. This blend is optimized for the production of targets <1kb in length. cDNA synthesis kit produces excellent results in both real time and conventional
RT-PCR.
- First strand cDNA synthesis for RT-PCR and real-time RT-PCR
- Synthesis of cDNA for cloning an expression.
- Generation of labelled cDNA probes for microarrays
- DNA labelling
- Analysis of RNA by primer extension