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Rat CK-MB (Creatine Kinase MB Isoenzyme) Quick Test ELISA Kit |
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Sensitivity |
18.75pg/ml |
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Range |
31.25-2000pg/ml |
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Alternative Name |
CKMB ELISA Kit, Creatine Kinase MB Isoenzyme ELISA Kit, CK-MB ELISA Kit |
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UniProt ID |
P07335 |
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Sample Type |
Serum, plasma, cell culture supernatant, cell lysate or tissue lysate, other biological fluid samples |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Wavelength |
OD450 |
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Reaction Duration |
120 minutes |
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Specificity |
Specifically binds with CKMB , no obvious cross reaction with other analogues. |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Principle |
This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains CKMB, a capture antibody-CKMB-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CKMB in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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