| KO-Validated GAPDH Recombinant Rabbit Monoclonal Antibody | |
| Description | KD-Validated Antibody |
| Conjugation | Unconjugated |
| Modification | Unmodified |
| Isotype | Rabbit IgG |
| Clonality | Rabbit mAb |
| Form | Liquid |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide. |
| Purification | Affinity Purification |
| Gene Name | GAPDH |
| Gene ID | 2597 |
| SwissProt ID | P04406 |
| Alternative Names | GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein; OCAS, P38 Component ; HEL-S-162eP; EC 2.6.99.-; EC 1.2.1; G3PD |
| Buffer | Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide. |
| Molecular Weight | Calculated MW: 36.1kDa |
| Dilution Ratio | WB 1:2,000-1:10,000; FC 1:200-1:2,000; IHC-P 1:250-1:500 |
| Research Area | Neuroscience,Isotype/Loading Controls,Signal Transduction,Cancer,Metabolism |
| Background | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
| Validated Image |
Western blotting analysis using GAPDH antibody (KVAb01827). GAPDH expression in wild type (WT) and GAPDH knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GAPDH antibody (KVAb01827, 1:100,000) and HRP-conjugated goat anti-rabbit secondary antibody (APS0635, 1:10,000) respectively 
Flow cytometric analysis of GAPDH expression in HepG2 cells using GAPDH antibody (KVAb01827, 1:2,000). Green, isotype control; red, GAPDH. |
KO-Validated GAPDH Recombinant Rabbit Monoclonal Antibody
| Catalogue No | BSAB-317P |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB,FCM,IHC-P |
| Size | 20μL | 50μL | 100μL |
| Price | POR |