|
GAPDH Rabbit mAb |
|
Product Type |
Loading Control Antibody |
|
Clone Number |
R08-4C3 |
|
Isotype |
IgG |
|
Purification |
Affinity Purified |
|
Gene Name |
GAPDH |
|
Immunogen |
Recombinant protein of human GAPDH |
|
Antibody type |
Monoclonal Antibody |
|
Recommended Dilutions |
WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200 |
|
Gene ID |
2597 |
|
Molecular Weight |
Calculated MW: 36 kDa; Observed MW: 36 kDa |
|
Swiss-Prot No |
P04406 |
|
Form of Antibody |
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
|
Storage/Stability |
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
|
Expression Host: |
CHO cell |
|
Background |
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types. |
|
Validated Image |
Immunohistochemistry analysis of paraffin-embedded (1) rat colon; (2) mouse testis; (3) Human uterus; (4) Human kidney, using GAPDH antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
|
