| PDGF-AA Protein, Human | |
| Appearance | Lyophilized powder |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Tag | Tag Free |
| Accession | P04085-1 (S87-T211) |
| Gene ID | 5154 |
| Synonyms | PDGFA; Platelet-Derived Growth Factor Alpha Chain; Platelet Derived Growth Factor Subunit A; Platelet-Derived Growth Factor A Chain; Platelet-Derived Growth Factor Alpha Polypeptide; PDGF Subunit A; PDGF1; PDGF A-Chain; Platelet-Derived Growth Factor Subu |
| Predicted Molecular Mass | 14.3 kDa |
| Molecular Weight | Approximately 16 kDa under reduced (R) conditions, 27-33 kDa under non reducing (N) conditions, based on SDS-PAGE, due to the glycosylation. |
| Purity | ≥ 95%, as determined by reducing SDS-PAGE. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). |
| Storage & Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
| Background | PDGF-A Protein takes center stage as a pivotal growth factor regulating embryonic development, cell proliferation, migration, survival, and chemotaxis. Acknowledged for its potent mitogenic effects on mesenchymal cells, PDGF-A is indispensable for various developmental processes, including the formation of lung alveolar septa, gastrointestinal tract development, Leydig cell maturation, spermatogenesis, and the normal development of oligodendrocytes, contributing to myelination in the spinal cord and cerebellum. Furthermore, PDGF-A assumes a crucial role in wound healing dynamics. The intricacies of its signaling pathway involve modulation through the formation of heterodimers with PDGFB, demonstrating its regulatory versatility. Structurally, PDGF-A adopts a homodimeric configuration with an antiparallel disulfide-linked dimer, and it forms heterodimers with PDGFB, engaging in interactions with PDGFRA homodimers and heterodimers formed by PDGFRA and PDGFRB. Additionally, PDGF-A exhibits an interaction with CSPG4, adding another layer to its multifaceted involvement in cellular processes |
| Validated Images |
≥ 95%, as determined by reducing SDS-PAGE.. 
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The ED50 for this effect is 7.548 ng/mL, corresponding to a specific activity is 1.324×105units/mg.. |
PDGF-AA Protein
| Catalogue No | BSRP-055 |
| Host | Human |
| Source | E. coli |
| Applications | - |
| Size | 2 μg | 10 μg |
| Price | POR |
PDGF-A protein is a key growth factor that regulates embryonic development, cell proliferation, migration, survival, and chemotaxis, and exerts potent mitogenic effects on mesenchymal cells. It is essential for alveolar septal formation, gastrointestinal tract development, interstitial cell maturation, spermatogenesis, and oligodendrocyte development, and contributes to spinal cord and cerebellar myelination. PDGF-AA Protein, Human is the recombinant human-derived PDGF-AA protein, expressed by E. coli , with tag free.
- Lyophilized from a 0.22 μm filtered solution of 20 mM Glycine-HCl, 6% sucrose, 4% mannitol, 0.02% Tween 80, pH 3.0
- Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, 200 mM arginine, pH 8.0.
- Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 20% Acetonitrile, pH 2.5.