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Lactate Dehydrogenase-Cytotoxicity Colorimetric Microplate Assay Kit |
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Description |
Detection and Quantification of cytotoxicity based on Lactate Dehydrogenase (LDH) released from damaged cells. |
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Detection Method |
Colorimetric |
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Sample Types |
Cell lysate, Cell culture media |
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Platform |
Microplate reader 490 nm |
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Storage/Stability |
Shipped and store at 4°C for 6 months. |
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Alternative Names |
EC 1.1.1.27; lactic acid dehydrogenase; LDH; L(+)-nLDH; L-(+)-lactate dehydrogenase; L-lactic dehydrogenase; L-lactic acid dehydrogenase; lactate dehydrogenase; lactate dehydrogenase NAD-dependent; lactic dehydrogenase; NAD-lactate dehydrogenase |
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Principle |
Cell death or cytotoxicity is evaluated by the quantification of plasma membrane Damage. The damage to cell membrane structures results in cytoplasmic enzymes being released into the culture medium. In these enzymes, Lactate Dehydrogenase (LDH) is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. Lactate Dehydrogenase-Cytotoxicity Colorimetric Microplate Assay Kit provides a simple and direct procedure for measuring lactate dehydrogenase activity released from damaged cells. In this colorimetric LDH quantification assay, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color. The rate of increase in the absorbency at 490 nm, is a measure of LDH activity. |
