| Lysine Decarboxylase Broth w/o Peptone (ISO Basis) | |
| Appearance (Form) | Free flowing, homogeneous powder |
| Appearance (Colour) | Greenish yellow |
| Solubility | 9.00 gms/lit |
| pH (25°C) | 6.60 - 7.00 |
| Prepared Medium Appearance (Colour) | Purple |
| Prepared Medium Appearance (Clarity) | Clear solution without any precipitate |
| Organism | Salmonella paratyphi A ATCC 9150 |
| Inoculum (cfu) 10-100 | Growth : Good, Lysine decarboxylase: negative reaction,yellow color |
| Organism | Salmonella paratyphi B ATCC 8759 |
| Inoculum (cfu) 10-100 | Growth : Good, Lysine decarboxylase: negative reaction,yellow color |
| Organism | Yersinia enterocolitica ATCC 27729 |
| Inoculum (cfu) 10-100 | Growth : Good, Lysine decarboxylase: negative reaction,yellow color |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum (cfu) 10-100 | Growth : Good, Lysine decarboxylase: negative reaction,yellow color |
| Cultural Response | Incubate the tubes (with caps tightened) at 35 ± 2°C for 18-48 hours or upto 96 hours before interpreting the results as negative. After the incubation period, observe the tubes for growth and decarboxylase reaction. In the initial stages of incubation the medium colour changes from purple to yellow due to fermentation of glucose by the organisms, with acid production. On further incubation, L-Lysine is decarboxylated to cadaverine, there will be an alkaline reaction (rise in pH) and the colour of the medium changes to purple. If colour remains yellow, the decarboxylase reaction is negative. |
| Composition | |
| Ingredients | Gram/Litre |
| L-Lysine monohydrochloride | 5.00 |
| Yeast extract | 3.00 |
| Glucose | 1.00 |
| Bromocresol Purple | 0.015 |
| pH (25°C) | 6.8 ± 0.2 |
Lysine Decarboxylase Broth w/o Peptone (ISO Basis)
| Catalouge No | BSDCM-16624 |
| HSN Code | 38210000 |
| Size | 100 GM | 500 GM |
For distinguishing Salmonella serotype arizonae from the Bethesda Ballerup group of Enterobacteriaceae.This medium is in accordance to specifications given by ISO 6579-1:2017 and ISO 10273:2003.