The AapCas12b nuclease (also known as C2c1) is a DNA endonuclease mediated by tracrRNA:crRNA (or sgRNA), derived from the thermophilic bacterium Alicyclobacillus acidophilus. In the presence of a PAM (TTN) sequence at the target double-stranded DNA (dsDNA), it specifically cleaves the target dsDNA, causing double-strand breaks and generating sticky ends. AapCas12b can specifically cleave single-stranded DNA targets independent of the PAM sequence. Both double-stranded and single-stranded DNA targets can activate the trans-cleavage activity of AapCas12b. When the AapCas12b enzyme forms a ternary complex with sgRNA and target DNA, it becomes activated for nonspecific ssDNA trans-cleavage activity, cutting any arbitrary sequence of ssDNA in the system. The optimal cleavage reaction temperature for AapCas12b is 60^oC, which makes it more heat-resistant than AacCas12b, and more suitable for use with LAMP to develop isothermal amplification/CRISPR-Cas detection systems.

Broad reaction temperature range: Exhibits cleavage activity within the temperature range of 37~65^oC

Low nuclease residue: No residual exonuclease, nickase, or RNase

Cis-cleavage activity: Highly Effective Cleavage of Double-Stranded DNA in Vitro

Trans-cleavage activity: High trans-cleavage activity, suitable for nucleic acid detection

• CRISPR/Cas gene editing
• Diagnostic and detection based on the CRISPR/Cas system
• Other detection applications combined with isothermal nucleic acid amplification technologies (RPA and LAMP), etc

Cis-cleavage Activity Verification of AapCas12b Nuclease

AapCas12b Nuclease Trans-cleavage Activity Test Results