| Hoechst 33342 trihydrochloride | |
| Synonyms | bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride |
| Appearance | Solid |
| Color | Light yellow to green yellow |
| IC50 & Target | Dye reagent | DNA Stain[ |
| SMILES | [H]Cl.[H]Cl.[H]Cl.CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1 |
| Shipping | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture and light, In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| Emission (Em) | 451 |
| Excitation (Ex) | 356 |
| Dyeing Example | Hoechst 33342 trihydrochloride can be used for nuclei staining with blue fluorescence. |
| Pack Size | 5 MG | 10 MG | 25 MG | 50 MG | 100 MG | 200 MG | 500 MG |
Hoechst 33342 Trihydrochloride
| Catalogue No | BSHY-15559A |
| Mol. Formula | C27H31Cl3N6O |
| Mol. Weight | 561.93 |
| Price | POR |
Hoechst 33342 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution
Labeling Nuclear DNA with Hoechst 33342[1] Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)