RIPA (Radio-Immuno Precipitation Assay) Buffer is a ready- to-use versatile solution and widely used lysis buffer for preparing cell lysates for a variety of protein-related applications. Radio Immuno Precipitation Assay buffer (RIPA buffer) is used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. RIPA lysis extraction buffer contains non-ionic and ionic detergents which are able to extract protein from wide variety of cell types and membrane structures
Protein lysis can be completed within 45 to 60 minutes.

RIPA buffer is compatible with a wide range of applications, including

• Western blotting
• Protein assays
• Immunoassays
• Protein purification

RIPA buffer ensures efficient cell lysis and protein solubilization Prevents protein degradation and interference with protein immunoreactivity and biological activity.

Minimizes non-specific protein-binding interactions to keep background low, Allows most specific interactions to occur, enabling studies of relevant protein-protein interactions.

RIPA buffer-conducted protein extraction is compatible with various downstream immunoprecipitation and molecular pull-down assays, including reporter assays, protein assays, immunoassays and protein purification.