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MagPure Particles

Biostring > Products > MagPure Particles
DNA-RNA-PROTEIN-KITS

MagPure Particles

Catalogue No Product Name Size Price
BSC-1410 BSC-14100 MagPure Particles 100 ML POR
BSC-14101 MagPure Particles 400 ML POR
BSC-14102 MagPure Particles 3 x 400 ML POR
BSC-14103 MagPure Particles 10 x 400 ML POR
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SKU: BSC-1410 Categories: ,
Features Specifications
Concentration 100 mg/ml
Appearance Suspension of black particles
Surface functional group Si-OH, Silanol
Dispersibility Polydisperse Amorphous
Particle size 1.5-5 μm
Preservation conditions Room Temperature, valid for up to 2 years. It is recommended to store in 2-8°C to prevent microbial growth.
Magnetic response speed 15-30 seconds
Settling velocity >5 minutes
High salt mediated binding >2M guanidine isothiocyanate, DNA recovery up to 80%
Alcohol mediated binding 2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85%
PEG8000 mediated binding The recovery of DNA/RNA was up to 85%
DNase/RNase Not detected
DNA residue <1 ppm
Recommended application Plasmid extraction, gel DNA recovery, genomic DNA extraction and RNA extraction.
MagPure Particles represent the standard silica-based magnetic bead system within the Magen sample preparation portfolio. The particles are designed for routine DNA and RNA purification from biological samples including tissue, blood and cultured cells. The system provides consistent adsorption behavior under chaotropic binding conditions and is compatible with a wide range of extraction buffers and workflows.

 

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.

MagPure Particles were evaluated in multiple nucleic acid binding systems, including high-salt, alcohol-mediated and guanidine isothiocyanate-based conditions. In DNA marker recovery tests, MagPure Particles showed stable recovery across different binding buffers, with recovered DNA concentrations of approximately 24.09–33.49 ng/µL under the tested conditions.

In agarose gel DNA recovery testing, MagPure Particles provided consistent recovery from both Buffer TE marker samples and 1.5% agarose gel samples. Compared with the other tested bead formats, MagPure Particles showed stable performance in gel-based DNA recovery workflows, supporting its use for gel extraction, product purification and routine DNA cleanup applications.

Application testing in genomic DNA extraction also showed that MagPure Particles can be used in blood, tissue and soil DNA workflows. Extracted DNA showed acceptable A260/280 and A260/230 values, and agarose gel electrophoresis confirmed recoverable genomic DNA bands, supporting its use as a general-purpose silica magnetic bead for nucleic acid purification.