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Histone H3 Rabbit Monoclonal Antibody |
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Description |
Recombinant Rabbit Monoclonal Antibody |
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Conjugation |
Unconjugated |
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Modification |
Unmodified |
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Isotype |
IgG,Kappa |
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Clonality |
Monoclonal |
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Form |
Liquid |
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Storage |
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
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Buffer |
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein |
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Purification |
Protein A |
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Gene Name |
HIST1H3A |
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Gene ID |
8350;8351;8352;8353;8354;8355;8356;8357;8358;8968 |
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SwissProt ID |
P68431 |
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Alternative Names |
HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3 |
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Dilution Ratio |
WB 1:5000-1:20000,IHC 1:5000-1:20000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200 |
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Molecular Weight |
Calculated MW:15kD;Observed MW:15kD |
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Background |
Cell localization:Nucleus.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
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Validated Image |
Western blot analysis of lysates from Hela cells, using Histone H3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody
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