|
PerCP-Cy5.5 Anti-Mouse CD3ε Antibody (145-2C11) |
|
Alternative Names |
CD3E| CD3e| T-cell surface antigen T3/Leu-4 epsilon chain| T-cell surface glycoprotein CD3 epsilon chain| T3E antibody |
|
Isotype |
IgG |
|
Form |
Liquid |
|
Concentration |
0.2mg/mL |
|
Conjugation |
PerCP-Cy5.5 |
|
Clonality |
Monoclonal |
|
Clone ID |
145-2C11 |
|
UniProt ID |
P22646 |
|
Conjugation Information |
PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). |
|
Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
|
Recommended dilution |
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume]. |
|
Background |
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
|
Validated Image |
C57BL/6 murine splenocytes are stained with PerCP-Cy5.5 Anti-Mouse CD3E Antibody (filled red histogram). Unstained splenocytes (empty black histogram) are used as control.
|
