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Human Cit (Citrulline) ELISA Kit |
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Sensitivity |
0.51 ng/mL |
|
Range |
1.57-100 ng/mL |
|
Detection Method |
Competitive Inhibition |
|
Sample Type |
serum, plasma, urine and other biological fluids |
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Alternative Names |
Citrulline |
|
Assay length |
2h |
|
Standard |
100 ng/mL |
|
Research Area |
Metabolism |
|
Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Cit. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Cit. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cit in the samples is then determined by comparing the OD of the samples to the standard curve. |
