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Human PF4 (Platelet Factor 4) ELISA Kit |
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Sensitivity |
0.38 ng/mL |
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Range |
0.63-40ng/mL |
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Alternative Names |
CXCL4,PF-4,SCYB4 |
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Detection Method |
Sandwich |
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Standard |
40ng/mL |
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Sample Type |
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL |
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Reaction time |
3.5H |
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Uniprot ID |
P02776 |
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Research Area |
Cardiovascular biology |
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Principle |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PF4. You can calculate the concentration of Human PF4 in the samples by comparing the OD of the samples to the standard curve. |
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Validated Image |
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Human PF4 (Platelet Factor 4) ELISA Kit |
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Sensitivity |
0.938ng/ml |
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Range |
1.563-100ng/ml |
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UniProt ID |
P02776 |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Detection Wavelength |
OD450 |
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Reaction Duration |
4 hours |
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Specificity |
Specifically binds with PF4 , no obvious cross reaction with other analogues. |
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Alternative Names |
Platelet factor 4 ELISA Kit, PF-4 ELISA Kit, C-X-C motif chemokine 4 ELISA Kit, Iroplact ELISA Kit, Oncostatin-A ELISA Kit, Platelet factor 4, short form ELISA Kit, Endothelial cell growth inhibitor ELISA Kit, PF4 ELISA Kit, CXCL4 ELISA Kit, SCYB4 ELISA Kit |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PF4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with PF4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of PF4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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