The HyPure Gel DNA Kit is designed for recovery of DNA fragments from agarose gels following electrophoresis. The workflow combines controlled gel dissolution conditions with silica membrane-based purification to ensure efficient DNA adsorption and reproducible recovery.
This system is typically used in cloning, sequencing and enzymatic workflows where removal of agarose and contaminants is required. The purification chemistry is optimized to maintain stable performance across different fragment sizes and routine laboratory conditions.
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.5g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA |
| Applications | PCR, NGS, labeling, ligation and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Agarose gel, PCR products, enzyme products |
| Sample amount | Agarose gel: ≤500mg |
| Recovery | ≥80% |
| Elution volume | ≥15μl |
| Time per run | ≤20 minutes（1-24 samples） |
| Liquid carrying volume | 800µl |
| Binding yield of column | 35µg |
Technical Validation
The HyPure Gel DNA Mini Kit was evaluated using DNA fragments recovered from agarose gels of different concentrations. Short DNA fragments of 150 bp and 500 bp were recovered from 2.5% agarose gel, medium fragments of approximately 1 kb and 5 kb were recovered from 1.2% agarose gel, and long fragments including 10 kb DNA and genomic DNA were recovered from 0.8% agarose gel. Gel analysis after purification showed successful recovery across the tested fragment-size range.
Comparative gel recovery testing was performed using the same DNA input and gel slice weight across different purification kits. The HyPure Gel DNA Mini Kit showed recovery performance comparable to commonly used commercial gel extraction workflows for short, medium and long DNA fragments, supporting its use for routine gel-based DNA purification.
Additional validation was performed using plasmid restriction digestion products mixed with agarose gel to simulate gel recovery conditions. Both long backbone fragments and short insert fragments were recovered efficiently, with gel band intensity indicating recovery above 60% and approaching 80% under the tested conditions. The Buffer GDP volume tested in the workflow did not show an obvious negative effect on recovery performance.