|
BHB (Beta-Hydroxybutyric Acid) ELISA Kit dtls |
|
Sensitivity |
0.46 µg/mL |
|
Range |
1.57-100 µg/mL |
|
Alternative Names |
OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid |
|
Detection Method |
Competitive Inhibition |
|
Standard |
100 µg/mL |
|
Sample Type |
serum, plasma, cell lysates, cell culture supernates and other biological fluids |
|
Reaction time |
2h |
|
Research Area |
Signal transduction;Metabolic pathway;Endocrinology |
|
Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with bHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of bHB in the samples is then determined by comparing the OD of the samples to the standard curve. |
|
Validated Image |
|
