Rat Bcl2 ELISA Kit
$450.00
| Catalogue No. | 5251ELK |
| Size | 96T |
| Alternative Names | B-Cell CLL/Lymphoma 2 |
| Reactivity | Rat |
| Range | 78.13-5000 pg/mL |
| Sensitivity | 28 pg/mL |
Description
| Product Name | Rat Bcl2(B-Cell Leukemia/Lymphoma 2) ELISA Kit | |||||||||||||||||||||||||||
| Catalogue No. | 7760ELK | |||||||||||||||||||||||||||
| Alternative Names: | B-Cell CLL/Lymphoma 2 | |||||||||||||||||||||||||||
| Assay Type | Sandwich | |||||||||||||||||||||||||||
| Sensitivity | 28 pg/mL | |||||||||||||||||||||||||||
| Range | 78.13-5000 pg/mL | |||||||||||||||||||||||||||
| Sample Type | serum, plasma, tissue homogenates and other biological fluids | |||||||||||||||||||||||||||
| Assay Length | 3.5h | |||||||||||||||||||||||||||
| Research Area | Signal transduction;Apoptosis; | |||||||||||||||||||||||||||
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Leukemia/Lymphoma 2(Bcl2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Cell Leukemia/Lymphoma 2(Bcl2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Leukemia/Lymphoma 2(Bcl2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell Leukemia/Lymphoma 2(Bcl2) in the samples is then determined by comparing the OD of the samples to the standard curve. | |||||||||||||||||||||||||||
| Standard Curve |
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| Precision | Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. |
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| Recovery | Matrices listed below were spiked with certain level of recombinant Bcl2 and the recovery rates were calculated by comparing the measured value to the expected amount of Bcl2 in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bcl2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| Note | For Research Use Only |
Additional information
| Pack Size | 96 Test |
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