Antibody Development
Producing a monoclonal/polyclonal antibody for research & Development and diagnostic application can be complicated and expensive. At Biostring we can make this process easy and seamless for you and provide excellent quality, cost effective, time efficient solution for developing your custom antibodies. We have a proven track record and extensive experience in handling challenging projects from industry and academia to develop monoclonal or polyclonal antibodies against various antigens such as Peptide, native protein, recombinant protein and small chemical structure.
If you have any questions or for additional information on Antibody Development Services, kindly contact us at
- info@bio-string.com
Description
Mouse Monoclonal Antibody Development (Cat# BS-MAB-201)
- Antigen analysis, Pre-screening, Immunization and Boosters
- Host: Mice
- Titer determination of antisera by ELISA
- Fusion of higher titer mouse
- Screening for positive clones by ELISA at 96 well stage to 24 well
- Single cell cloning of selected clones, characterization by ELISA
- Isotype determination of Selected reactive clones
- One cycle of freeze/ thaw stability check for final selected clones
- Antibody purification from best reactive clone (~1 mg) Additional Service
Rabbit Polyclonal Antibody/Antisera Development (Cat# BS-PAB-301)
- Antigen analysis, Pre-screening and Immunization and Boosters
- Host: New Zealand White Rabbits (2 Nos.)
- Titer determination of antisera by ELISA
- Pre-immune Antisera: 0.3 ML
- Hyperimmune Antisera: 40 ML per host
- Purity determination by SDS-PAGE and quantification by standard methods
Goat Polyclonal Antibody/Antisera Development Against Protein (Cat# BS-PAB-302)
- Antigen analysis, Pre-screening and Immunization and Boosters.
- Host: Goat (1 Nos.)
- Titer determination of antisera by ELISA.
- Hyperimmune antisera: 1000 ML
Guinea pig Polyclonal Antibody/Antisera Development (Cat# BS-PAB-303)
- Antigen analysis, Pre-screening and Immunization and Boosters.
- Host: Guinea Pig (2 Nos.)
- Titer determination of antisera by ELISA
- Hyperimmune antisera: 10 ML per Host
Mouse Polyclonal Antibody/Antisera Development (Cat# BS-PAB-405)
- Antigen analysis, Pre-screening and Immunization and Boosters
- Titer determination of antisera by ELISA
- Host: Rat (2 Nos.)
- Hyperimmune Antisera: 5 ML
Mouse Polyclonal Antibody/Antisera Development (Cat# BS-PAB-304)
- Antigen analysis, Pre-screening and Immunization and Boosters
- Titer determination of antisera by ELISA
- Host: Mice (2 Nos.)
- Hyperimmune Antisera: 1 ML
Antigen Affinity Purification
- Custom affinity purification of antibody from antisera (Cat# BS-IM-206)
Antibody /Protein Labelling Service
- Antibody reactivity to antigen by western blot (Cat# BS-IM-207)
- Labelling of antibody/Protein to HRP and Quality determination by ELISA (Cat# BS-IM-208)
- Labelling of antibody to Biotin and Quality determination by ELISA (Cat# BS-IM-209)
- Labelling of antibody to ALP and Quality determination by ELISA (Cat# BS-IM-210)
- Labelling of antibody to FITC and Quality determination by ELISA (Cat# BS-IM-211)
- Gold nanoparticle labelling to antibody (Cat# BS-IM-212)
- KLH/BSA conjugation to peptide (Cat# BS-IM-213)
- by papain digestion antibody and purification of Fab fragment (BS-IM-214)
- Antibody Match Pair Selection for ELISA Application; (Cat# BS-MAB-202)
- Ascitic fluid generation in mice; (Cat# BS-MAB-204)
- Purification of antibody from mice ascitic fluid; (Cat#BS-MAB-205)
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