Product Name |
Mouse KL-6(Krebs Von den Lungen-6) ELISA Kit |
Catalogue No. |
5860ELK |
Synonyms |
KL-6; Mucin-1; MUC1 |
Assay Type |
Sandwich |
Sensitivity |
32.1 pg/mL |
Range |
78.13-5000 pg/mL |
Sample Type |
serum, plasma, tissue homogenates and other biological fluids |
Assay Length |
3.5h |
Research Area |
Interstitial lung disease |
Test principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Krebs Von den Lungen-6(KL-6). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Krebs Von den Lungen-6(KL-6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Krebs Von den Lungen-6(KL-6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Krebs Von den Lungen-6(KL-6) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve |
Concentration (ng/mL) |
OD |
Corrected OD |
5000.00 |
2.165 |
2.070 |
2500.00 |
1.694 |
1.599 |
1250.00 |
1.219 |
1.124 |
625.00 |
0.864 |
0.769 |
312.50 |
0.567 |
0.472 |
156.25 |
0.318 |
0.223 |
78.13 |
0.196 |
0.101 |
0.00 |
0.095 |
0.000 |
|
Precision |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. |
Recovery |
Matrices listed below were spiked with certain level of recombinant KL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of KL-6 in samples.
Matrix |
Recovery range |
Average |
serum(n=5) |
83-95% |
89% |
EDTA plasma(n=5) |
96-107% |
102% |
Heparin plasma(n=5) |
95-104% |
98% |
|
Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
91-101% |
87-98% |
89-103% |
81-96% |
EDTA plasma(n=5) |
98-107% |
93-102% |
80-93% |
91-100% |
Heparin plasma(n=5) |
92-105% |
85-101% |
85-94% |
87-96% |
|
Note |
For Research Use Only |
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