PanProbes One-Step RT-qPCR Kit delivers high sensitivity of the target RNA level due to its RScript reverse transcriptase, a reduced RNase H+ activity MMLV enzyme in addition to a powerful RNase inhibitors mix which aim to diminish RNA degradation and mis priming during reaction setup and reverse transcription to guarantee optimal RT efficiency.

The Universal qPCR Master Mix is a 2x concentrated, ready-to-use master mix optimized for probe-based real-time PCR and compatible with the majority of commercially available real-time PCR systems (ROX-independent and ROX-dependent). It contains antibody-mediated hot-start Taq DNA polymerase, dNTPs, MgCl2, enhancers, stabilizers and essentials for a success PCR reaction

To evaluate the performance, the Ribonuclease P (RP)gene with varying RNA template concentrations, through serial dilutions, were used. Based on the fluorescence value of the RP probe, the results show the performance corresponding to the Ct value with different copy numbers.

Figure – Performance of PanProbes One-Step RT-qPCR Master Mix

Kit Content(s)

2X Universal qPCR Master Mix : 1 ml x 1 vial
RScript Enzyme Mix : 20 μl x 1 vial

Instrument Compatibility

This Master Mix is compatible with the majority of commercially available real-time PCR systems.