Description
| Product Name | Mouse KL-6(Krebs Von den Lungen-6) ELISA Kit | |||||||||||||||||||||||||||
| Catalogue No. | 5860ELK | |||||||||||||||||||||||||||
| Synonyms | KL-6; Mucin-1; MUC1 | |||||||||||||||||||||||||||
| Assay Type | Sandwich | |||||||||||||||||||||||||||
| Sensitivity | 32.1 pg/mL | |||||||||||||||||||||||||||
| Range | 78.13-5000 pg/mL | |||||||||||||||||||||||||||
| Sample Type | serum, plasma, tissue homogenates and other biological fluids | |||||||||||||||||||||||||||
| Assay Length | 3.5h | |||||||||||||||||||||||||||
| Research Area | Interstitial lung disease | |||||||||||||||||||||||||||
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Krebs Von den Lungen-6(KL-6). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Krebs Von den Lungen-6(KL-6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Krebs Von den Lungen-6(KL-6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Krebs Von den Lungen-6(KL-6) in the samples is then determined by comparing the OD of the samples to the standard curve. | |||||||||||||||||||||||||||
| Standard Curve |
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| Precision | Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. |
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| Recovery | Matrices listed below were spiked with certain level of recombinant KL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of KL-6 in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| Note | For Research Use Only |
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