| Catalogue No. |
0327HE |
| Size |
96T |
| Synonyms |
VEGF ELISA Kit, VAS ELISA Kit, Vasculotropin ELISA Kit, VEGFA ELISA Kit, VPF ELISA Kit, MVCD1 ELISA Kit, vascular endothelial growth factor A ELISA Kit, Vascular permeability factor ELISA Kit, VEGF-A ELISA Kit, VPFvascular endothelial growth factor ELISA Kit |
| Species |
Human |
| Range |
31.25-2000pg/ml |
| Sensitivity |
< 18.75pg/ml |
| Detection Method |
Sandwich ELISA, Double Antibody |
| Application |
VEGF ELISA Kit allows for the in vitro quantitative determination of VEGF concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Standard Curve |
 |
| Storage |
2-8 ℃ for 6 months |
| Recovery |
Matrices listed below were spiked with certain level of VEGF and the recovery rates were calculated by comparing the measured value to the expected amount of VEGF in samples.
| Matrix |
Recovery range(%) |
Average(%) |
| serum(n=5) |
88-104 |
96 |
| EDTA plasma(n=5) |
85-101 |
92 |
| heparin plasma(n=5) |
85-104 |
98 |
|
| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample |
1:2 |
1:4 |
1:8 |
| serum(n=5) |
90-105% |
89-105% |
85-105% |
| EDTA plasma(n=5) |
83-98% |
82-101% |
82-96% |
| heparin plasma(n=5) |
91-95% |
83-99% |
80-94% |
|
| CV(%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
| Note |
For Research Use Only |
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