The Rituximab ELISA kit is designed to quantify / measure the Rituximab with high specificity and sensitivity in biological matrices.

Test Principle

The DeQuanto® Rituximab (Mabthera® ) PK ELISA kit is a sandwich immunoassay. The Rituximab present in the sample binds to pre coated Anti-Rituximab Antibody on polystyrene microtiter plate. Non bound or the excess substances are removed by washing. Horseradish Peroxidase (HRP) labeled antibody is added to the wells. The plate is washed to remove any unbound antibody_x0002_enzyme reagent and substrate solution is added to the wells. A color product is formed in proportion to the amount of Rituximab present in the sample or standards. The color development is stopped by addition of a stop solution. The absorbance is measured at 450 nm in a microtiter plate reader. The concentration of Rituximab in a sample can be interpolated from the standard
curve.