Rituximab (Mabthera®) immunogenicity ELISA kit is intended for qualitative determination of antibodies to Rituximab in human serum/plasma.

Test Principle

The DeQuanto® Rituximab (Mabthera®) immunogenicity ELISA kit is an ELISA based immunoassay. The antibodies to Rituximab present in the sample binds to pre coated Rituximab on polystyrene microtiter plate. Non bound or the excess substances are removed by washing. Horseradish Peroxidase (HRP) labeled Rituximab is added to the wells, which bind to the Anti Rituximab antibody in the micro_x0002_wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the micro-wells. A color product is formed in proportion to the amount of Anti Rituximab Antibody present in the sample. The color development is stopped by addition of a stop solution. The absorbance is measured at 450 nm in a microtiter plate reader.