Adalimumab (Humira®) Immunogenicity ELISA kit can be efficiently used for monitoring the Anti Adalimumab antibodies to adalimumab in human serum/plasma.

Test Principle

The DeQuanto® Anti Adalimumab (Humira®) immunogenicity ELISA kit is an ELISA based immunoassay. The antibodies to Adalimumab present in the sample binds to pre coated Adalimumab on polystyrene microtiter plate. Non bound or the excess substances are removed by washing. Horseradish Peroxidase (HRP) labeled Adalimumab is added to the wells, which bind to the Anti Adalimumab antibody in the micro-wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the micro-wells. A color product is formed in proportion to the amount of Anti Adalimumab Antibody present in the sample. The color development is stopped by addition of a stop solution. The absorbance is measured at 450 nm in a microtiter plate reader.