Description
Species
Human
Assay Length
3h
Test Method
Competitive Inhibition
Sensitivity
The minimum detectable dose of this kit is typically less than 1.35ng/mL.
Detection Range
3.70-300ng/mL
Sample type
Serum, plasma and other biological fluids
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitrotyrosine (NT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitrotyrosine (NT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Matrices listed below were spiked with certain level of Nitrotyrosine (NT) and the recovery rates were calculated by comparing the measured value to the expected amount of Nitrotyrosine (NT) in samples.
Matrix
Recovery Range(%)
Average (%)
serum(n=5)
83-105
| 91 |
EDTA plasma(n=5)
98-105
| 101 |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nitrotyrosine (NT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
90-97%
80-89%
78-94%
80-99%
EDTA plasma(n=5)
86-101%
80-103%
81-93%
79-90%
Reviews
There are no reviews yet.