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Product name |
Arg(Arginine) ELISA Kit |
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Alternative Names |
(S)-2-Amino-5-Guanidinopentanoic Acid |
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Assay Type |
Competitive Inhibition |
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Standard |
100 µg/mL |
|
Sample Type |
serum, plasma, other biological fluids |
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Assay Length |
2h |
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Research Area |
Metabolic pathway;Nutrition metabolism |
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Test Principle |
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This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Arg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arg in the samples is then determined by comparing the OD of the samples to the standard curve. |
